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@#【Abstract】 【Objective】To investigate the differential expression of long non-coding RNA-1708(lncRNA-1708)in osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSC)and its effect on osteogenic differentiation of hBMSC.【Methods】Purchased 3 groups of hBMSC from different sources and cultured in vitro. qRT-PCR was used to detect the expression of lncRNA-1708 after osteogenic differentiation of three groups of hBMSC,and the relationship between lncRNA-1708 and hBMSC osteogenic differentiation was analyzed. LncRNA-1708 overexpressing lentivirus and lncRNA-1708 interferenced plasmid were transfected respectively,to obtain stable hBMSC cell line. After 14-day osteogenic differentiation on transfected hBMSC,RT-PCR was used to detect the expressions of runt-related transcription factor 2(RUNX2)and alkaline phosphatase(ALP)mRNA,and alkaline phosphatase staining was made.【Results】The expression of lnc-1708 decreased after osteogenic differentiation of hBMSC for 7 d(P < 0. 001).Two cell lines,which respectively express high lncRNA-1708 and low lncRNA-1708,were constructed successfully. In lnc-1708-overexpressed BMSC,the mRNA levels of osteogenic markers RUNX2 and ALP were both significantly down-regulated(0.46±0.03 vs.1.00±0.02,0.15±0.07 vs. 1.02±0.28,P < 0.01). On the contrary,in the lnc-1708-silencing BMSC,the expressions of RUNX2 and ALP mRNA level were significantly up-regulated(1.62±0.18 vs. 1.00±0.04,1.58±0.11 vs. 1.01±0.18,P < 0.01).【Conclusion】LncRNA-1708 may have an inhibitory effect on osteogenic differentiation of hBMSC.
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Objective To investigate the association of DNA methyltransfcrase 3B (DNMT3B) promoter region single nucleo tide polymorphism (SNP)-149C＞T and-579G＞T with genetic susceptibility of esophageal cancer (EC) of Han population in Jiangsu Suqian.Methods Gcnotypes of the-149C＞T and 579G＞T locus in DNMT3B promoter region were examinedby polymerase chain reaction with restriction fragment length polymorphism (PCR RFLP) in 246 esophageal cancer patients and 240 healthy controls.Results In-149C＞T site,the distributions of genotypes TT compared with CT in the EC group and the controls were no significant difference (x2 =0.089,P＞0.05).In-579G＞T site,the distributions of genotypes TT compared with GT+GG in the EC group and the controls were no significant difference (x2 =0.649,P＞0.05).When strat ified by age and gender,there was no significant difference in the EC group and the controls in both two sites (x2 =0.044～0.876,all P＞0.05).Conclusion The DNMT3B promoter region-149C＞T and-579G＞T were not associated with the genetic susceptibility of esophageal cancer.
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[Objective]To analyze the expression profile variation of circle RNA(circRNA)in tongue squamous cell carcinoma(TSCC)tissue and para-carcinoma tissue.[Methods]CircRNA microarray technology was performed to in-spect the difference of circRNA expression in 4 cases of TSCC tissues and 4 cases of para-carcinoma tissues,and then make analysis after the quality control and homogenization of raw data,to identify which have more than 2 times variation and significant difference(P<0.05)by statistical analysis as circRNA with differential expression. To perform functional analysis on circRNA with differential expression.[Results]Compared with para-carcinoma tissue,there were 17171 circRNA differentially expressed in TSCC tissue,while 9982 increase more than 2 times and 7189 reduce more than 2 times.[Conclusion]circRNA expression profile in TSCC changes significantly comparing with the para-carcinoma tis-sue,some differentially expressed circRNA may regulate the occurrence and progression of TSCC through a competitive combination of miRNA.
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[Objectives]To observe the clinical effects of the immediate implant placement of Zimmer dental implant sys-tem in the mandibular posterior region.[Methods]67 cases with a total of 89 mandible posterior teeth to deal with immediate implantion which selected to be treated with Zimmer dental implant system.76 teeth between implant and tooth socket bone wall were simultaneously filled with Bio-oss Collagen.The upper structure was repaired with PFM porcelain crown after the postoperative phase I of 3~6 months.All the patients were followed up for 6~24 months.[Results]During the clinical follow up,the implant survival rate was 100%. In 67 patients,89 implants was successfully loaded,with stable implants,good condition in synosteosis and without adverse subjective symptoms. All of the 67 patients had achieved good synosteosis and success loads clinically and radiologically.[Conclusion]A good osseointegration is obtained in the mandibular posterior re-gion with Zimmer dental implant system,Correctly dealing with Bio-oss Collagen between implant and tooth socket bone wall.At the same time,it can shorten the time of therapy,simplify the procedure,so that the clinical results are more satis-factory.
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<p><b>OBJECTIVE</b>To detect CCR5 protein expression in different human tongue squamous cell carcinoma cells and observe the effect of macrophage inflammatory protein-1β (MIP-1β) on the proliferation and apoptosis of CAL-27 cells.</p><p><b>METHODS</b>Western blotting and immunofluorescence staining were used to detect the expression of the CCR5, the receptor of MIP-1β, in 3 human tongue squamous cell carcinoma cells UM-1, CAL-27, and Tca-8113. CCK-8 assay was used to assess the proliferation of CAL-27 cells stimulated with 10, 20, and 40 ng/mL MIP-1β for 12, 24, or 48 h. The apoptosis of the cells stimulated with MIP-1β (10, 20, and 40 ng/mL) for 24 h was analyzed using flow cytometry with Annexin V/PI double staining.</p><p><b>RESULTS</b>CCR5 expression was detected both on the membrane and in the cytoplasm in all the 3 tongue squamous cell carcinoma cell lines. At the concentrations of 10, 20, and 40 ng/mL, MIP-1β stimulation for 12 and 24 h significantly promoted the proliferation of CAL-27 cells (P<0.05); MIP-1β stimulation for 48 h at the concentrations 10 and 20 ng/mL, but not at 40 ng/mL, promoted the proliferation of CAL-27 cells (P<0.05). MIP-1β stimulation at 40 ng/mL for 24 produced the most obvious apoptosis-inducing effect in CAL -27 cells (P<0.05), while MIP-1β at 10 or 20 ng/mL did not induce obvious apoptosis in the cells (P>0.05).</p><p><b>CONCLUSION</b>CCR5 is expressed in all the 3 human tongue squamous cell carcinoma cells. MIP-1β can promote the proliferation of CAL-27 cells but high concentrations of MIP-1β also induced cell apoptosis. Prolonged stimulation of the cells with a high concentration of MIP-1β shows attenuated effect in promoting cell proliferation probably as a result of cell apoptosis induced by MIP-1β.</p>
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<p><b>OBJECTIVE</b>To study if quantum dots fluorescent probes can be applied to detect P53 protein and Bcl-2 protein in tissue of human tongue squamous cell carcinoma.</p><p><b>METHODS</b>By indirect immunofluorescence assay the same particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein respectively. Different particle size quantum dots fluorescent probes were applied to detect P53 protein and Bcl-2 protein simultaneously in paraffin-embedded tissue section of human tongue squamous cell carcinoma under fluorescent microscope.</p><p><b>RESULTS</b>P53 protein and Bcl-2 protein can be combined with quantum dots fluorescent probes and specific fluorescene can be observed with ultraviolet light excited. P53 protein was mainly distributed in the nucleus, and Bcl-2 protein major in the cytoplasm. P53 protein and Bcl-2 protein can be combined with different particle size quantum dots fluorescent probes respectively in the same paraffin-embedded tissue section of human tongue squamous cell carcinoma and two kinds of fluorescene can be observed.</p><p><b>CONCLUSION</b>Quantum dots fluorescent probes can be applied to detect two kinds of specific protein in paraffin-embedded tissue section of human tongue squamous cell carcinoma.</p>
Subject(s)
Humans , Carcinoma, Squamous Cell , Fluorescent Dyes , Quantum Dots , Tongue Neoplasms , Tumor Suppressor Protein p53ABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to investigate the clinical effective of immediate restoration with Osstem MS mini-implant in the edentulous space of 5-6 mm.</p><p><b>METHODS</b>The sample consisted of 36 consecutively treated partially edentulous patients who had a total of 36 Osstem MS mini-implants, which were 2.5 mm or 3.0 mm in diameter and placed in 5-6 mm gap. The chair-side-made or laboratory-made provisional crowns for implants were fabricated at the time of fixtures placed. The final restorations were fabricated with gold alloy-fused-porcelain crown 3 to 5 months later. During the mean 21.3 months (12-37 months) follow-up time since fixtures placement, all implants were examined clinically and radiologically.</p><p><b>RESULTS</b>No implant failed before restoration. One implant led an adjacent tooth pulp necrosis after the implantation, but the natural tooth and implant were successfully retained by root canal therapy. 36 implants in 36 patients who were followed-up were successful and their aesthetic results were satisfactory.</p><p><b>CONCLUSION</b>Immediate loaded implant with Osstem MS mini-implant has good clinical prosthetic effects in the edentulous space of 5-6 mm.</p>
Subject(s)
Humans , Dental Abutments , Dental Implantation, Endosseous , Dental Prosthesis, Implant-Supported , Jaw, EdentulousABSTRACT
<p><b>OBJECTIVE</b>To explore a computer aided design (CAD) and rapid prototyping (RP) approach for fabrication of complete denture and to develop relevant programs for implementing it.</p><p><b>METHODS</b>Automatic crossing section scanner was used to scan artificial teeth and 3D graphic database of artificial teeth that could be aligned with parameters was established. A 3D laser scanner was used to scan upper and lower edentulous jaw casts and rims made in clinic. The vertical and horizontal relations were recorded before scanning with a patient instrument. Based on Imageware 11, tooth-arrangement curves, coordinate system, and landmark points for positioning were created, and construction cure and shape-controlling curve for base plate were constructed as well. Three-dimensional integrated design of complete denture, including artificial tooth automatic arrangement, aesthetic and individualized design of base plate, and artificial gingival, were finished. The programs were developed following the approach and the CAD platform was established. The virtual molds of complete dentures were constructed according to the above data in design and RP technology was used to make the plaster molds. Finally, the teeth were inserted and the complete denture was finished by dental technician.</p><p><b>RESULTS</b>The approach for the complete denture CAD/RP was confirmed and the CAD software platform was developed. A complete denture was manufactured.</p><p><b>CONCLUSIONS</b>The rules for complete denture in textbooks were expressed in design process with the CAD program developed by researcher. The 3D data of rims were utilized in design so that the digital, intelligentized and individualized design and manufacture process for complete denture was implemented.</p>
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Humans , Computer-Aided Design , Denture, Complete , Software DesignABSTRACT
<p><b>OBJECTIVE</b>This study was to explore a CAD method for constructing occlusal and proximal surfaces of inlays using reverse engineering (RE) software and to develop a CAD software for the inlay fabrication.</p><p><b>METHODS</b>The dentition model of a volunteer with individual normal occlusion was scanned with a 3D mechanical scanner. The scanned objects were prepared teeth with G. V. Black class I, class II and class VI (MOD) cavities, neighbor teeth, intercuspal position (ICP) record, and functional bite record (or functional generate path, FGP). Inlays were constructed based on RE technology using the database of Chinese normal teeth.</p><p><b>RESULTS</b>The technique guideline of inlay CAD was developed based on RE softwares. Inlays designed with CAD technique showed smooth and continuous contours. The occlusal and proximal contacts of inlay met physiologic demands.</p><p><b>CONCLUSIONS</b>It is a feasible method to develop a CAD software for inlay fabrication based on RE software.</p>